Exhibit 8.1
List of Principal Subsidiaries
| Name of Subsidiaries | Jurisdiction of Incorporation | |
| Yisheng US Biopharma Inc. | United States | |
| LakeShore Biopharma (Singapore) Pte. Ltd. | Singapore | |
| YishengBio (Hong Kong) Holdings Limited | Hong Kong | |
| LakeShore Tech Hong Kong Limited | Hong Kong | |
| Liaoning Yisheng Biopharma Co., Ltd. | PRC | |
| Beijing Yisheng Biotechnology Co., Ltd. | PRC | |
| Hu’an Yuanhang Biotechnology (Beijing) Co., Ltd. | PRC | |
| YS BIOPHARMA (PHILIPPINES) INC. | Philippines |